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發酵缸廠家

想要知道發酵缸廠家的正確使用方法嗎?請看下面的內容介紹。1、校正pH電極和溶氧電極。2、罐體**。根據需要將培養基配入罐體,按要…

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想要知道發酵缸廠家的正確使用方法嗎?請看下面的內容介紹。

發酵缸廠家

1、校正pH電極和溶氧電極。

2、罐體滅菌。根據需要將培養基配入罐體,按要求封好后,小型抄發酵罐(5L)可將罐體放入大滅菌鍋滅菌(115℃,30分鐘);大型發酵罐(10L及以上)采用蒸汽滅菌(121℃,30min)。

3、待罐體冷卻后,將其置于發酵臺上,安裝完好;打開冷卻水,打開氣泵電源,連接通氣管道開始通氣,調節進氣旋鈕使通氣量適當,使罐壓保持在0.05MPa;設置溫度、pH、攪拌速度等,并在確定的轉速和通氣量下對溶氧電極進行斜率標定,數值為百分之百。

4、待溫度穩定,各項參數都正確后,將預搖好的種子接入,開始發酵計時,并開始記錄各種參數。

5、發酵完畢后清洗罐zhidao體和電極,將pH電極插入有3M氯化鉀的三角瓶中待用,溶氧電極的探頭用保護套套好,保存備用。


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